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This page was generated on 2016-09-21 03:38:02 -0700 (Wed, 21 Sep 2016).
Package 585/1257 | Hostname | OS / Arch | INSTALL | BUILD | CHECK | BUILD BIN | |||||
HTSeqGenie 4.3.0 Jens Reeder
| zin1 | Linux (Ubuntu 16.04 LTS) / x86_64 | NotNeeded | OK | [ ERROR ] | ||||||
moscato1 | Windows Server 2008 R2 Standard (64-bit) / x64 | ...NOT SUPPORTED...NOT SUPPORTED...NOT SUPPORTED... | |||||||||
morelia | Mac OS X Mavericks (10.9.5) / x86_64 | ...NOT SUPPORTED...NOT SUPPORTED...NOT SUPPORTED... |
Package: HTSeqGenie |
Version: 4.3.0 |
Command: /home/biocbuild/bbs-3.4-bioc/R/bin/R CMD check --no-vignettes --timings HTSeqGenie_4.3.0.tar.gz |
StartedAt: 2016-09-20 07:55:45 -0700 (Tue, 20 Sep 2016) |
EndedAt: 2016-09-20 08:19:57 -0700 (Tue, 20 Sep 2016) |
EllapsedTime: 1452.4 seconds |
RetCode: 1 |
Status: ERROR |
CheckDir: HTSeqGenie.Rcheck |
Warnings: NA |
############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/bbs-3.4-bioc/R/bin/R CMD check --no-vignettes --timings HTSeqGenie_4.3.0.tar.gz ### ############################################################################## ############################################################################## * using log directory ‘/home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck’ * using R version 3.3.1 (2016-06-21) * using platform: x86_64-pc-linux-gnu (64-bit) * using session charset: UTF-8 * using option ‘--no-vignettes’ * checking for file ‘HTSeqGenie/DESCRIPTION’ ... OK * checking extension type ... Package * this is package ‘HTSeqGenie’ version ‘4.3.0’ * checking package namespace information ... OK * checking package dependencies ... OK * checking if this is a source package ... OK * checking if there is a namespace ... OK * checking for hidden files and directories ... NOTE Found the following hidden files and directories: .BBSoptions These were most likely included in error. See section ‘Package structure’ in the ‘Writing R Extensions’ manual. * checking for portable file names ... OK * checking for sufficient/correct file permissions ... OK * checking whether package ‘HTSeqGenie’ can be installed ... OK * checking installed package size ... NOTE installed size is 5.0Mb sub-directories of 1Mb or more: test-data 3.6Mb * checking package directory ... OK * checking ‘build’ directory ... OK * checking DESCRIPTION meta-information ... NOTE Malformed Title field: should not end in a period. * checking top-level files ... OK * checking for left-over files ... OK * checking index information ... OK * checking package subdirectories ... OK * checking R files for non-ASCII characters ... OK * checking R files for syntax errors ... OK * checking whether the package can be loaded ... OK * checking whether the package can be loaded with stated dependencies ... OK * checking whether the package can be unloaded cleanly ... OK * checking whether the namespace can be loaded with stated dependencies ... OK * checking whether the namespace can be unloaded cleanly ... OK * checking loading without being on the library search path ... OK * checking dependencies in R code ... NOTE Unexported objects imported by ':::' calls: ‘ShortRead:::.set_omp_threads’ ‘gmapR:::..gsnap’ ‘gmapR:::GeneGenome’ ‘gmapR:::checkPackageInstalled’ ‘gmapR:::getDefaultGmapGenomePath’ ‘gmapR:::getGeneRoi’ ‘gmapR:::subsetRegion’ ‘parallel:::mckill’ See the note in ?`:::` about the use of this operator. * checking S3 generic/method consistency ... OK * checking replacement functions ... OK * checking foreign function calls ... NOTE Foreign function call to a different package: .Call(ShortRead:::.set_omp_threads, ...) See chapter ‘System and foreign language interfaces’ in the ‘Writing R Extensions’ manual. * checking R code for possible problems ... NOTE .makePreprocessAlignPlots: no visible binding for global variable ‘data’ .plot.in.out.min.max.readlength: no visible global function definition for ‘median’ FastQStreamer.init: no visible binding for '<<-' assignment to ‘FastQStreamer.lfqs’ FastQStreamer.init: no visible binding for '<<-' assignment to ‘FastQStreamer.chunkid’ FastQStreamer.init: no visible binding for '<<-' assignment to ‘FastQStreamer.subsampling_filter’ FastQStreamer.init: no visible binding for '<<-' assignment to ‘FastQStreamer.max_nbchunks’ addHandler: no visible binding for '<<-' assignment to ‘logging.handlers’ addHandler: no visible binding for global variable ‘logging.handlers’ addHandler: no visible binding for '<<-' assignment to ‘logging.file’ buildAnyFastaGenome: no visible global function definition for ‘getSeq’ buildGenomicFeaturesFromTxDb: no visible global function definition for ‘gaps’ buildGenomicFeaturesFromTxDb: no visible global function definition for ‘ranges’ buildTP53FastaGenome: no visible global function definition for ‘getSeq’ buildTP53GenomeTemplate: no visible global function definition for ‘path’ buildTallyParam: no visible global function definition for ‘seqinfo’ computeCoverage: no visible global function definition for ‘resize’ computeCoverage: no visible global function definition for ‘coverage’ estimateCutoffs : <anonymous>: no visible binding for global variable ‘quantile’ getGenomeSegments: no visible global function definition for ‘seqinfo’ hashVariants: no visible global function definition for ‘ranges’ isAboveQualityThresh: no visible global function definition for ‘Views’ isAboveQualityThresh: no visible global function definition for ‘viewMeans’ listIterator.init: no visible binding for '<<-' assignment to ‘listIterator.n’ listIterator.init: no visible binding for '<<-' assignment to ‘listIterator.x’ logReset: no visible binding for '<<-' assignment to ‘logging.handlers’ logReset: no visible binding for '<<-' assignment to ‘logging.loglevel’ logReset: no visible binding for '<<-' assignment to ‘logging.file’ loglevel: no visible binding for global variable ‘logging.loglevel’ loglevel: no visible binding for global variable ‘logging.handlers’ plotDF : .plot: no visible global function definition for ‘plot’ processChunks : tracefun: no visible binding for global variable ‘sjobs’ processChunks : tracefun: no visible binding for global variable ‘chunkid’ setLevel: no visible binding for '<<-' assignment to ‘logging.loglevel’ statCountFeatures: no visible global function definition for ‘quantile’ truncateReads: no visible global function definition for ‘subseq’ vcfStat: no visible global function definition for ‘quantile’ writeToFile: no visible binding for global variable ‘logging.file’ Undefined global functions or variables: Views chunkid coverage data gaps getSeq logging.file logging.handlers logging.loglevel median path plot quantile ranges resize seqinfo sjobs subseq viewMeans Consider adding importFrom("graphics", "plot") importFrom("stats", "median", "quantile") importFrom("utils", "data") to your NAMESPACE file. * checking Rd files ... OK * checking Rd metadata ... OK * checking Rd cross-references ... OK * checking for missing documentation entries ... WARNING Undocumented code objects: ‘annotateVariants’ All user-level objects in a package should have documentation entries. See chapter ‘Writing R documentation files’ in the ‘Writing R Extensions’ manual. * checking for code/documentation mismatches ... OK * checking Rd \usage sections ... OK * checking Rd contents ... OK * checking for unstated dependencies in examples ... OK * checking sizes of PDF files under ‘inst/doc’ ... OK * checking installed files from ‘inst/doc’ ... OK * checking files in ‘vignettes’ ... OK * checking examples ... OK * checking for unstated dependencies in ‘tests’ ... OK * checking tests ... Running ‘doRUnit.R’ ERROR Running the tests in ‘tests/doRUnit.R’ failed. Last 13 lines of output: test.sclapply: ERROR !! Error in mcfork(detached) : unable to fork, possible reason: Cannot allocate memory test.tryKeepTraceback: (3 checks) ... OK (0.01 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.trimReads.R test.truncateReads: (7 checks) ... OK (0.03 seconds) test.truncateReads.trim5: (15 checks) ... OK (0.1 seconds) Error in eval(expr, envir, enclos) : runTests.R: failed to run unit tests, see /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/tests/HTSeqGenie-runit.txt for details Calls: source -> withVisible -> eval -> eval Execution halted * checking for unstated dependencies in vignettes ... OK * checking package vignettes in ‘inst/doc’ ... OK * checking running R code from vignettes ... SKIPPED * checking re-building of vignette outputs ... SKIPPED * checking PDF version of manual ... OK * DONE Status: 1 ERROR, 1 WARNING, 6 NOTEs See ‘/home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/00check.log’ for details.
doRUnit.Rout.fail:
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair" Copyright (C) 2016 The R Foundation for Statistical Computing Platform: x86_64-pc-linux-gnu (64-bit) R is free software and comes with ABSOLUTELY NO WARRANTY. You are welcome to redistribute it under certain conditions. Type 'license()' or 'licence()' for distribution details. R is a collaborative project with many contributors. Type 'contributors()' for more information and 'citation()' on how to cite R or R packages in publications. Type 'demo()' for some demos, 'help()' for on-line help, or 'help.start()' for an HTML browser interface to help. Type 'q()' to quit R. > library("HTSeqGenie") Loading required package: gmapR Loading required package: GenomeInfoDb Loading required package: BiocGenerics Loading required package: parallel Attaching package: 'BiocGenerics' The following objects are masked from 'package:parallel': clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport, clusterMap, parApply, parCapply, parLapply, parLapplyLB, parRapply, parSapply, parSapplyLB The following objects are masked from 'package:stats': IQR, mad, xtabs The following objects are masked from 'package:base': Filter, Find, Map, Position, Reduce, anyDuplicated, append, as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table, tapply, union, unique, unsplit, which, which.max, which.min Loading required package: S4Vectors Loading required package: stats4 Attaching package: 'S4Vectors' The following objects are masked from 'package:base': colMeans, colSums, expand.grid, rowMeans, rowSums Loading required package: IRanges Loading required package: GenomicRanges Loading required package: Rsamtools Loading required package: Biostrings Loading required package: XVector Loading required package: ShortRead Loading required package: BiocParallel Loading required package: GenomicAlignments Loading required package: SummarizedExperiment Loading required package: Biobase Welcome to Bioconductor Vignettes contain introductory material; view with 'browseVignettes()'. To cite Bioconductor, see 'citation("Biobase")', and for packages 'citation("pkgname")'. Loading required package: VariantAnnotation Attaching package: 'VariantAnnotation' The following object is masked from 'package:base': tabulate > > source(getPackageFile("unitTests/runTests.R")) Loading required package: GenomicFeatures Loading required package: AnnotationDbi did not source anything in dirname= ./R Executing test function test.alignReads ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 07:59:20 INFO::preprocessReads.R/preprocessReads: starting... 2016-09-20 07:59:20 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 07:59:20 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq 2016-09-20 07:59:20 DEBUG::tools.R/processChunks: starting... 2016-09-20 07:59:21 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 07:59:21 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/chunks/chunk_000001/logs/progress.log 2016-09-20 07:59:23 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.043 minutes 2016-09-20 07:59:23 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/chunks/chunk_000002/logs/progress.log 2016-09-20 07:59:26 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.043 minutes 2016-09-20 07:59:26 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/chunks/chunk_000003/logs/progress.log 2016-09-20 07:59:29 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.043 minutes 2016-09-20 07:59:29 DEBUG::tools.R/processChunks: done 2016-09-20 07:59:29 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 07:59:29 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/results/test_pe.adapter_contaminated_1.RData 2016-09-20 07:59:29 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ... 2016-09-20 07:59:29 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/results/test_pe.adapter_contaminated_2.RData 2016-09-20 07:59:29 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75 2016-09-20 07:59:29 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/results/test_pe.summary_preprocess.tab 2016-09-20 07:59:29 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/bams/processed.aligner_input_1.fastq ... 2016-09-20 07:59:29 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/bams/processed.aligner_input_2.fastq ... 2016-09-20 07:59:29 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/reports/shortReadReport_1 ... 2016-09-20 07:59:30 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/reports/shortReadReport_2 ... 2016-09-20 07:59:32 INFO::preprocessReads.R/preprocessReads: done 2016-09-20 07:59:32 INFO::alignReads.R/alignReads: starting alignment... 2016-09-20 07:59:32 DEBUG::tools.R/processChunks: starting... 2016-09-20 07:59:33 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 07:59:33 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/chunks/chunk_000001/logs/progress.log 2016-09-20 07:59:37 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.067 minutes 2016-09-20 07:59:37 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/chunks/chunk_000002/logs/progress.log 2016-09-20 07:59:39 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.045 minutes 2016-09-20 07:59:39 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/chunks/chunk_000003/logs/progress.log 2016-09-20 07:59:42 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.045 minutes 2016-09-20 07:59:42 DEBUG::tools.R/processChunks: done 2016-09-20 07:59:42 INFO::alignReads.R/mergeBAMsAcrossDirs: starting... 2016-09-20 07:59:42 INFO::alignReads.R/mergeBAMsAcrossDirs: done 2016-09-20 07:59:42 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/results/test_pe.summary_alignment.tab 2016-09-20 07:59:42 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/results/test_pe.summary_analyzed_bamstats.tab 2016-09-20 07:59:42 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report... 2016-09-20 07:59:42 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.79f5263be62b/results/test_pe.summary_target_lengths.tab 2016-09-20 07:59:43 INFO::reportPipelineQA.R/writePreprocessAlignReport: done 2016-09-20 07:59:43 INFO::alignReads.R/alignReads: done done successfully. Executing test function test.alignReads.sparsechunks ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 07:59:44 INFO::preprocessReads.R/preprocessReads: starting... 2016-09-20 07:59:44 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 07:59:44 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 2 , totnbreads= 26 ) 2016-09-20 07:59:44 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 07:59:44 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq 2016-09-20 07:59:44 DEBUG::tools.R/processChunks: starting... 2016-09-20 07:59:45 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 07:59:45 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/chunks/chunk_000001/logs/progress.log 2016-09-20 07:59:48 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.042 minutes 2016-09-20 07:59:48 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/chunks/chunk_000002/logs/progress.log 2016-09-20 07:59:50 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.042 minutes 2016-09-20 07:59:50 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/chunks/chunk_000003/logs/progress.log 2016-09-20 07:59:53 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.043 minutes 2016-09-20 07:59:53 DEBUG::tools.R/processChunks: starting chunkid= 4 ; see logfile= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/chunks/chunk_000004/logs/progress.log 2016-09-20 07:59:56 DEBUG::tools.R/processChunks: done with chunkid= 4 ; elapsed.time= 0.042 minutes 2016-09-20 07:59:56 DEBUG::tools.R/processChunks: done 2016-09-20 07:59:56 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 07:59:56 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/results/test_pe.adapter_contaminated_1.RData 2016-09-20 07:59:56 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ... 2016-09-20 07:59:56 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/results/test_pe.adapter_contaminated_2.RData 2016-09-20 07:59:56 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=2 highqual_reads=1 adapter_contam=0 read_length=75 rRNA_contam_reads=0 processed_reads=1 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75 2016-09-20 07:59:56 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/results/test_pe.summary_preprocess.tab 2016-09-20 07:59:56 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/bams/processed.aligner_input_1.fastq ... 2016-09-20 07:59:56 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/bams/processed.aligner_input_2.fastq ... 2016-09-20 07:59:56 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/reports/shortReadReport_1 ... 2016-09-20 07:59:57 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.alignReads.sparsechunks.79f562faf7ab/reports/shortReadReport_2 ... 2016-09-20 07:59:58 INFO::preprocessReads.R/preprocessReads: done done successfully. Executing test function test.alignReadsOneSingleEnd ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:00 INFO::alignReads.R/alignReadsChunk: running gsnap... 2016-09-20 08:00:00 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -D /home/biocbuild/.local/share/gmap -t 1 -d TP53_demo_3.2.2 --quality-protocol=illumina -A sam --read-group-id=test_se -m 0 --split-output /tmp/Rtmp8SYYBa/test.alignReadsOneSingleEnd.79f57eed7cc5/bams/test.alignReads /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2>&1 2016-09-20 08:00:00 INFO::alignReads.R/createSummaryAlignment: counting unique bam reads... 2016-09-20 08:00:00 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReadsOneSingleEnd.79f57eed7cc5/results/test.alignReads.summary_alignment.tab 2016-09-20 08:00:00 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.alignReadsOneSingleEnd.79f57eed7cc5/results/test.alignReads.summary_analyzed_bamstats.tab 2016-09-20 08:00:00 INFO::alignReads.R/alignReadsChunk: done done successfully. Executing test function test.annotateVariants ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:02 INFO::analyzeVariants/annotateVariants: starting... Timing stopped at: 1.836 0.316 7.996 Error in annotateVariants(vcf) : variant_effect_predictor.pl failed.--vcf --sift b --polyphen b --protein --check_existing --check_alleles --symbol --canonical --ccds --maf_1kg --maf_esp --cache --force_overwrite --no_stats --fork 2 --input_file /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/braf_v600.vcf --output_file /tmp/Rtmp8SYYBa/test.annotateVariants.79f52a878016/results/test_pe.variants_vep.vcf done successfully. Executing test function test.callVariantsVariantTools.genotype ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:10 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.genotype.79f53df5557b/results/test_pe.coverage.RData 2016-09-20 08:00:10 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp8SYYBa/test.genotype.79f53df5557b/results/test_pe.coverage.bw 2016-09-20 08:00:10 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.genotype.79f53df5557b/results/test_pe.summary_coverage.tab 2016-09-20 08:00:10 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:00:11 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:00:11 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:00:11 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:00:11 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.genotype.79f53df5557b/results/test_pe.raw_variants.RData 2016-09-20 08:00:11 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.genotype.79f53df5557b/results/test_pe.filtered_variants.RData 2016-09-20 08:00:11 INFO::analyzeVariants.R/wrap.callVariants: ...done 2016-09-20 08:00:11 INFO::analyzeVariants.R/writeVCF: writing vcf file... 2016-09-20 08:00:11 INFO::analyzeVariants.R/writeVCF: ...done 2016-09-20 08:00:11 INFO::analyzeVariants.R/.callGenotypes: calling genotypes... 2016-09-20 08:00:37 INFO::analyzeVariants.R/writeVCF: writing vcf file... 2016-09-20 08:00:37 INFO::analyzeVariants.R/writeVCF: ...done 2016-09-20 08:00:37 INFO::analyzeVariants.R/.callGenotypes: done done successfully. Executing test function test.wrap.callVariants ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:39 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:00:40 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:00:40 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:00:40 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:00:40 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.79f516cec76b/results/test_pe.raw_variants.RData 2016-09-20 08:00:40 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.79f516cec76b/results/test_pe.filtered_variants.RData 2016-09-20 08:00:40 INFO::analyzeVariants.R/wrap.callVariants: ...done done successfully. Executing test function test.wrap.callVariants.filters ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:41 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:00:42 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:00:42 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:00:42 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:00:42 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.filters.79f519f2eb1c/results/test_pe.raw_variants.RData 2016-09-20 08:00:42 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.filters.79f519f2eb1c/results/test_pe.filtered_variants.RData 2016-09-20 08:00:42 INFO::analyzeVariants.R/wrap.callVariants: ...done 2016-09-20 08:00:42 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:00:43 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:00:43 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:00:43 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:00:43 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.filters.79f519f2eb1c/results/test_pe.raw_variants.RData 2016-09-20 08:00:43 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.filters.79f519f2eb1c/results/test_pe.filtered_variants.RData 2016-09-20 08:00:43 INFO::analyzeVariants.R/wrap.callVariants: ...done done successfully. Executing test function test.wrap.callVariants.parallel ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:45 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:00:48 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:00:49 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:00:49 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:00:49 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.parallel.79f5156e421f/results/test_pe.raw_variants.RData 2016-09-20 08:00:49 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.parallel.79f5156e421f/results/test_pe.filtered_variants.RData 2016-09-20 08:00:49 INFO::analyzeVariants.R/wrap.callVariants: ...done done successfully. Executing test function test.wrap.callVariants.which ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:50 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:00:50 INFO::analyzeVariants.R/buildTallyParam: restricting variant calls using 'which' 2016-09-20 08:00:50 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:00:50 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:00:51 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:00:51 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.which.79f5113d2913/results/test_pe.raw_variants.RData 2016-09-20 08:00:51 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.wrap.callVariants.which.79f5113d2913/results/test_pe.filtered_variants.RData 2016-09-20 08:00:51 INFO::analyzeVariants.R/wrap.callVariants: ...done done successfully. Executing test function test.writeVCF.NULL ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:52 INFO::analyzeVariants.R/writeVCF: writing vcf file... 2016-09-20 08:00:52 INFO::analyzeVariants.R/writeVCF: ...done done successfully. Executing test function test.writeVCF.vcfStat ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:00:53 INFO::analyzeVariants.R/writeVCF: writing vcf file... 2016-09-20 08:00:53 INFO::analyzeVariants.R/writeVCF: ...done done successfully. Executing test function test.isFirstFragment ... done successfully. Executing test function test.buildCountsGRangesList ... done successfully. Executing test function test.generateSingleGeneDERs ... done successfully. Executing test function test.computeCoverage ... done successfully. Executing test function test.isSparse ... done successfully. Executing test function test.mergeCoverage ... 2016-09-20 08:16:22 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/vbkpxlgw/merged/results/bla.coverage.RData 2016-09-20 08:16:22 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp8SYYBa/vbkpxlgw/merged/results/bla.coverage.bw 2016-09-20 08:16:23 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/vbkpxlgw/merged/results/bla.summary_coverage.tab done successfully. Executing test function test.mergeCoverage.sparse ... 2016-09-20 08:16:24 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/gjdybclw/merged/results/bla.coverage.RData 2016-09-20 08:16:24 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp8SYYBa/gjdybclw/merged/results/bla.coverage.bw 2016-09-20 08:16:24 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/gjdybclw/merged/results/bla.summary_coverage.tab done successfully. Executing test function test.checkConfig.analyzeVariants ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt done successfully. Executing test function test.findTemplate ... done successfully. Executing test function test.checkConfig ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt done successfully. Executing test function test.checkConfig.alignReads ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt done successfully. Executing test function test.getConfig ... done successfully. Executing test function test.loadConfig ... done successfully. Executing test function test.parseDCF ... done successfully. Executing test function test.updateConfig ... done successfully. Executing test function test.getAdapterSeqs ... done successfully. Executing test function test.isAdapter ... done successfully. Executing test function test.isAdapter3.primeEnd ... done successfully. Executing test function test.detectRRNA ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:16:40 INFO::detectRRNA.R/detectRRNA: searching for rRNA contamination... 2016-09-20 08:16:40 INFO::io.R/writeFastQFiles: writing filename= /tmp/Rtmp8SYYBa/test.detectRRNA.79f51f2d41ce/bams/rRNA_contam/input1.fastq 2016-09-20 08:16:40 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp8SYYBa/test.detectRRNA.79f51f2d41ce/bams/rRNA_contam/test_se /tmp/Rtmp8SYYBa/test.detectRRNA.79f51f2d41ce/bams/rRNA_contam/input1.fastq 2>&1 2016-09-20 08:16:40 INFO::detectRRNA.R/detectRRNA: contaminated fraction= 1 2016-09-20 08:16:40 INFO::detectRRNA.R/detectRRNA: done done successfully. Executing test function test.detectRRNA.paired_end ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:16:43 INFO::detectRRNA.R/detectRRNA: searching for rRNA contamination... 2016-09-20 08:16:43 INFO::io.R/writeFastQFiles: writing filename= /tmp/Rtmp8SYYBa/test.detectRRNA.paired_end.79f57f8b0362/bams/rRNA_contam/input1.fastq 2016-09-20 08:16:43 INFO::io.R/writeFastQFiles: writing filename= /tmp/Rtmp8SYYBa/test.detectRRNA.paired_end.79f57f8b0362/bams/rRNA_contam/input2.fastq 2016-09-20 08:16:43 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp8SYYBa/test.detectRRNA.paired_end.79f57f8b0362/bams/rRNA_contam/test_pe /tmp/Rtmp8SYYBa/test.detectRRNA.paired_end.79f57f8b0362/bams/rRNA_contam/input1.fastq -a paired /tmp/Rtmp8SYYBa/test.detectRRNA.paired_end.79f57f8b0362/bams/rRNA_contam/input2.fastq 2>&1 2016-09-20 08:16:44 INFO::detectRRNA.R/detectRRNA: contaminated fraction= 1 2016-09-20 08:16:44 INFO::detectRRNA.R/detectRRNA: done done successfully. Executing test function test.getRRNAIds ... 2016-09-20 08:16:44 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp8SYYBa/test_get_rRNA_idsmixclfrh/test_pe /tmp/Rtmp8SYYBa/test_get_rRNA_idsmixclfrh/1.fastq -a paired /tmp/Rtmp8SYYBa/test_get_rRNA_idsmixclfrh/2.fastq 2>&1 done successfully. Executing test function test.getRRNAIds_random ... 2016-09-20 08:16:45 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp8SYYBa/test_get_rRNAIds_randomnapmwesh/test_pe /tmp/Rtmp8SYYBa/test_get_rRNAIds_randomnapmwesh/1.fastq 2>&1 done successfully. Executing test function test.filterByLength ... 2016-09-20 08:16:47 INFO::filterQuality.R/filterQuality: filterByLength... 2016-09-20 08:16:47 INFO::filterQuality.R/filterByLength: fraction of filtered short reads= 0.5 2016-09-20 08:16:47 INFO::filterQuality.R/filterByLength: done 2016-09-20 08:16:47 INFO::filterQuality.R/filterQuality: filterByLength... 2016-09-20 08:16:47 INFO::filterQuality.R/filterByLength: fraction of filtered short reads= 1 2016-09-20 08:16:47 INFO::filterQuality.R/filterByLength: done done successfully. Executing test function test.isAboveQualityThresh ... done successfully. Executing test function test.trimTailsByQuality ... 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ... 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: done 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ... 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: done 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ... 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: done 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ... 2016-09-20 08:16:48 INFO::preprocessReads.R/preprocessReadsChunk: done done successfully. Executing test function test.callVariantsGATK ... Timing stopped at: 0 0 0 Error in DEACTIVATED("callVariantsGATK() tests need gatk.path option set") : callVariantsGATK() tests need gatk.path option set In addition: Warning message: closing unused connection 3 (/tmp/Rtmp8SYYBa/template_test_config.txt) done successfully. Executing test function test.callVariantsGATK.withFiltering ... Timing stopped at: 0 0 0 Error in DEACTIVATED("callVariantsGATK() tests need gatk.path option set") : callVariantsGATK() tests need gatk.path option set done successfully. Executing test function test.checkGATKJar ... Timing stopped at: 0 0 0 Error in DEACTIVATED("checkGATKJar() test needs gatk.path option set") : checkGATKJar() test needs gatk.path option set done successfully. Executing test function test.excludeVariantsByRegion ... done successfully. Executing test function test.gatk ... Timing stopped at: 0 0 0 Error in DEACTIVATED("gatk() tests need gatk.path option set") : gatk() tests need gatk.path option set done successfully. Executing test function test.realignIndels ... Timing stopped at: 0 0 0 Error in DEACTIVATED("test.realignIndels() tests need gatk.path option set") : test.realignIndels() tests need gatk.path option set done successfully. Executing test function test.realignIndelsGATK ... Timing stopped at: 0 0 0 Error in DEACTIVATED("realignIndelsGATK() tests need gatk.path option set") : realignIndelsGATK() tests need gatk.path option set done successfully. Executing test function test.realignIndelsGATK.parallel ... Timing stopped at: 0.004 0 0 Error in DEACTIVATED("realignIndelsGATK() tests need gatk.path option set") : realignIndelsGATK() tests need gatk.path option set done successfully. Executing test function test_zipUp ... done successfully. Executing test function test.FastQStreamer.getReads.pefq ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:16:55 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:16:55 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq done successfully. Executing test function test.FastQStreamer.getReads.pefq.subsample ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:16:57 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:16:57 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 17 , totnbreads= 26 ) 2016-09-20 08:16:57 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:16:57 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq done successfully. Executing test function test.FastQStreamer.getReads.segz ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:17:00 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/reads.fastq.gz done successfully. Executing test function test.FastQStreamer.getReads.truncated ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:17:02 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/truncated.fastq.gz done successfully. Executing test function test.FastQStreamer.subsampler.isdeterministic ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:17:05 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:17:05 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 17 , totnbreads= 26 ) 2016-09-20 08:17:05 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:17:05 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:17:07 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:17:07 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 17 , totnbreads= 26 ) 2016-09-20 08:17:07 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:17:07 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq done successfully. Executing test function test.createTmpDir ... done successfully. Executing test function test.detectQualityInFASTQFile ... done successfully. Executing test function test.getObjectFilename ... done successfully. Executing test function test.safeUnlink ... done successfully. Executing test function test.writeAudit ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt done successfully. Executing test function test.mergeLanes ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:17:18 INFO::preprocessReads.R/preprocessReads: starting... 2016-09-20 08:17:18 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:17:18 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq 2016-09-20 08:17:18 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:17:20 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:17:20 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000001/logs/progress.log 2016-09-20 08:17:22 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.045 minutes 2016-09-20 08:17:22 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000002/logs/progress.log 2016-09-20 08:17:25 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.045 minutes 2016-09-20 08:17:25 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000003/logs/progress.log 2016-09-20 08:17:28 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.044 minutes 2016-09-20 08:17:28 DEBUG::tools.R/processChunks: done 2016-09-20 08:17:28 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 08:17:28 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.adapter_contaminated_1.RData 2016-09-20 08:17:28 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ... 2016-09-20 08:17:28 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.adapter_contaminated_2.RData 2016-09-20 08:17:28 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75 2016-09-20 08:17:28 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.summary_preprocess.tab 2016-09-20 08:17:28 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/bams/processed.aligner_input_1.fastq ... 2016-09-20 08:17:28 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/bams/processed.aligner_input_2.fastq ... 2016-09-20 08:17:28 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/reports/shortReadReport_1 ... 2016-09-20 08:17:30 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/reports/shortReadReport_2 ... 2016-09-20 08:17:32 INFO::preprocessReads.R/preprocessReads: done 2016-09-20 08:17:33 INFO::alignReads.R/alignReads: starting alignment... 2016-09-20 08:17:33 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:17:34 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:17:34 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000001/logs/progress.log 2016-09-20 08:17:37 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.051 minutes 2016-09-20 08:17:37 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000002/logs/progress.log 2016-09-20 08:17:40 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.051 minutes 2016-09-20 08:17:40 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000003/logs/progress.log 2016-09-20 08:17:43 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.051 minutes 2016-09-20 08:17:43 DEBUG::tools.R/processChunks: done 2016-09-20 08:17:43 INFO::alignReads.R/mergeBAMsAcrossDirs: starting... 2016-09-20 08:17:43 INFO::alignReads.R/mergeBAMsAcrossDirs: done 2016-09-20 08:17:43 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.summary_alignment.tab 2016-09-20 08:17:43 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.summary_analyzed_bamstats.tab 2016-09-20 08:17:43 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report... 2016-09-20 08:17:44 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.summary_target_lengths.tab 2016-09-20 08:17:44 INFO::reportPipelineQA.R/writePreprocessAlignReport: done 2016-09-20 08:17:44 INFO::alignReads.R/alignReads: done 2016-09-20 08:17:44 INFO::countGenomicFeatures.R/countGenomicFeatures: starting... 2016-09-20 08:17:44 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:17:45 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:17:45 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000001/logs/progress.log 2016-09-20 08:17:48 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.041 minutes 2016-09-20 08:17:48 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000002/logs/progress.log 2016-09-20 08:17:50 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.041 minutes 2016-09-20 08:17:50 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000003/logs/progress.log 2016-09-20 08:17:53 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.041 minutes 2016-09-20 08:17:53 DEBUG::tools.R/processChunks: done 2016-09-20 08:17:53 INFO::countGenomicFeatures.R/mergeCounts: starting... 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.counts_exon.tab 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.counts_gene.tab 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.counts_gene_coding.tab 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.counts_gene_exonic.tab 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.counts_intergenic.tab 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.counts_intron.tab 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.counts_transcript.tab 2016-09-20 08:17:53 INFO::countGenomicFeatures.R/mergeCounts: done 2016-09-20 08:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.summary_counts.tab 2016-09-20 08:17:53 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: creating report of genomic features counts... 2016-09-20 08:17:53 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: done 2016-09-20 08:17:53 INFO::countGenomicFeatures.R/countGenomicFeatures: done... 2016-09-20 08:17:53 INFO::coverage.R/calculateCoverage: starting... 2016-09-20 08:17:54 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:17:55 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:17:55 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000001/logs/progress.log 2016-09-20 08:17:57 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.037 minutes 2016-09-20 08:17:57 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000002/logs/progress.log 2016-09-20 08:17:59 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.037 minutes 2016-09-20 08:17:59 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/chunks/chunk_000003/logs/progress.log 2016-09-20 08:18:01 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.037 minutes 2016-09-20 08:18:01 DEBUG::tools.R/processChunks: done 2016-09-20 08:18:02 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.coverage.RData 2016-09-20 08:18:02 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.coverage.bw 2016-09-20 08:18:02 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.summary_coverage.tab 2016-09-20 08:18:02 INFO::coverage.R/calculateCoverage: done 2016-09-20 08:18:02 INFO::analyzeVariants/analyzeVariants: starting ... 2016-09-20 08:18:02 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:18:04 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:18:04 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:18:04 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:18:04 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.raw_variants.RData 2016-09-20 08:18:04 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.filtered_variants.RData 2016-09-20 08:18:04 INFO::analyzeVariants.R/wrap.callVariants: ...done 2016-09-20 08:18:04 INFO::analyzeVariants.R/writeVCF: writing vcf file... 2016-09-20 08:18:04 INFO::analyzeVariants.R/writeVCF: ...done 2016-09-20 08:18:04 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/results/test_pe.summary_variants.tab 2016-09-20 08:18:04 INFO::analyzeVariants/analyzeVariants: done 2016-09-20 08:18:04 INFO::Pipeline run successful. 2016-09-20 08:18:06 INFO::mergeLanes.R/doMergeLanes: starting... 2016-09-20 08:18:06 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 08:18:06 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.adapter_contaminated_1.RData 2016-09-20 08:18:06 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ... 2016-09-20 08:18:06 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.adapter_contaminated_2.RData 2016-09-20 08:18:06 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75 2016-09-20 08:18:06 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.summary_preprocess.tab 2016-09-20 08:18:06 INFO::alignReads.R/mergeBAMsAcrossDirs: starting... 2016-09-20 08:18:06 INFO::alignReads.R/mergeBAMsAcrossDirs: done 2016-09-20 08:18:06 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.summary_alignment.tab 2016-09-20 08:18:06 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.summary_analyzed_bamstats.tab 2016-09-20 08:18:06 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report... 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.summary_target_lengths.tab 2016-09-20 08:18:07 INFO::reportPipelineQA.R/writePreprocessAlignReport: done 2016-09-20 08:18:07 INFO::countGenomicFeatures.R/mergeCounts: starting... 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.counts_exon.tab 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.counts_gene.tab 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.counts_gene_coding.tab 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.counts_gene_exonic.tab 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.counts_intergenic.tab 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.counts_intron.tab 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.counts_transcript.tab 2016-09-20 08:18:07 INFO::countGenomicFeatures.R/mergeCounts: done 2016-09-20 08:18:07 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.summary_counts.tab 2016-09-20 08:18:07 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: creating report of genomic features counts... 2016-09-20 08:18:07 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: done 2016-09-20 08:18:08 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.coverage.RData 2016-09-20 08:18:08 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.coverage.bw 2016-09-20 08:18:08 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.mergeLanes.79f553c19cd0/merged/results/merged.summary_coverage.tab 2016-09-20 08:18:08 INFO::analyzeVariants/analyzeVariants: starting ... 2016-09-20 08:18:08 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:18:08 ERROR::tools.R/safeExecute: caught exception: 2016-09-20 08:18:08 ERROR::Error in open.connection(file, "rt"): cannot open the connection 2016-09-20 08:18:08 ERROR::tools.R/safeExecute: traceback: 2016-09-20 08:18:09 ERROR::38: open.connection(file, "rt") 2016-09-20 08:18:09 ERROR::37: open(file, "rt") 2016-09-20 08:18:09 ERROR::36: read.table(.get_genome(path(directory(x)), genome(x), chromosomes = TRUE), 2016-09-20 08:18:09 ERROR::35: suppressWarnings({ 2016-09-20 08:18:09 ERROR::34: seqinfo(genome) 2016-09-20 08:18:09 ERROR::33: seqinfo(genome) 2016-09-20 08:18:09 ERROR::32: normArgWhich(which, params$genome) 2016-09-20 08:18:09 ERROR::31: (function (genome, which = GRanges(), desired_read_group = NULL, 2016-09-20 08:18:09 ERROR::30: do.call(BamTallyParam, bam.tally.args) 2016-09-20 08:18:09 ERROR::29: do.call(BamTallyParam, bam.tally.args) 2016-09-20 08:18:09 ERROR::28: (function (genome, read_pos_breaks = NULL, high_base_quality = 0L, 2016-09-20 08:18:09 ERROR::27: do.call(TallyVariantsParam, args) 2016-09-20 08:18:09 ERROR::26: do.call(TallyVariantsParam, args) 2016-09-20 08:18:09 ERROR::25: buildTallyParam() 2016-09-20 08:18:09 ERROR::24: wrap.callVariants(bam.file) 2016-09-20 08:18:09 ERROR::23: callVariantsVariantTools(bam.file) 2016-09-20 08:18:09 ERROR::22: eval(expr, env) 2016-09-20 08:18:09 ERROR::21: try(eval(expr, env), silent = TRUE) 2016-09-20 08:18:09 ERROR::20: safeExecute({ 2016-09-20 08:18:09 ERROR::19: analyzeVariants() 2016-09-20 08:18:09 ERROR::18: eval(expr, env) 2016-09-20 08:18:09 ERROR::17: try(eval(expr, env), silent = TRUE) 2016-09-20 08:18:09 ERROR::16: safeExecute({ 2016-09-20 08:18:09 ERROR::15: doMergeLanes(indirs) 2016-09-20 08:18:09 ERROR::14: eval(expr, env) 2016-09-20 08:18:09 ERROR::13: try(eval(expr, env), silent = TRUE) 2016-09-20 08:18:09 ERROR::12: safeExecute({ 2016-09-20 08:18:09 ERROR::11: mergeLanes(indirs, mergedir, prepend_str, num_cores = 1, preMergeChecks.do = FALSE, 2016-09-20 08:18:09 ERROR::10: func() 2016-09-20 08:18:09 ERROR::9: system.time(func()) 2016-09-20 08:18:09 ERROR::8: try(system.time(func())) 2016-09-20 08:18:09 ERROR::7: .executeTestCase(funcName, envir = sandbox, setUpFunc = .setUp, 2016-09-20 08:18:09 ERROR::6: .sourceTestFile(testFile, testSuite$testFuncRegexp) 2016-09-20 08:18:09 ERROR::5: runTestSuite(testSuite) 2016-09-20 08:18:09 ERROR::4: eval(expr, envir, enclos) 2016-09-20 08:18:09 ERROR::3: eval(ei, envir) 2016-09-20 08:18:09 ERROR::2: withVisible(eval(ei, envir)) 2016-09-20 08:18:09 ERROR::1: source(getPackageFile("unitTests/runTests.R")) 2016-09-20 08:18:09 ERROR::tools.R/safeExecute: caught exception: 2016-09-20 08:18:09 ERROR::Error in open.connection(file, "rt"): cannot open the connection 2016-09-20 08:18:09 ERROR::tools.R/safeExecute: traceback: 2016-09-20 08:18:09 ERROR::22: (function (e) 2016-09-20 08:18:09 ERROR::21: stop(z$e) 2016-09-20 08:18:09 ERROR::20: safeExecute({ 2016-09-20 08:18:09 ERROR::19: analyzeVariants() 2016-09-20 08:18:09 ERROR::18: eval(expr, env) 2016-09-20 08:18:09 ERROR::17: try(eval(expr, env), silent = TRUE) 2016-09-20 08:18:09 ERROR::16: safeExecute({ 2016-09-20 08:18:09 ERROR::15: doMergeLanes(indirs) 2016-09-20 08:18:09 ERROR::14: eval(expr, env) 2016-09-20 08:18:09 ERROR::13: try(eval(expr, env), silent = TRUE) 2016-09-20 08:18:09 ERROR::12: safeExecute({ 2016-09-20 08:18:09 ERROR::11: mergeLanes(indirs, mergedir, prepend_str, num_cores = 1, preMergeChecks.do = FALSE, 2016-09-20 08:18:09 ERROR::10: func() 2016-09-20 08:18:09 ERROR::9: system.time(func()) 2016-09-20 08:18:09 ERROR::8: try(system.time(func())) 2016-09-20 08:18:09 ERROR::7: .executeTestCase(funcName, envir = sandbox, setUpFunc = .setUp, 2016-09-20 08:18:09 ERROR::6: .sourceTestFile(testFile, testSuite$testFuncRegexp) 2016-09-20 08:18:09 ERROR::5: runTestSuite(testSuite) 2016-09-20 08:18:09 ERROR::4: eval(expr, envir, enclos) 2016-09-20 08:18:09 ERROR::3: eval(ei, envir) 2016-09-20 08:18:09 ERROR::2: withVisible(eval(ei, envir)) 2016-09-20 08:18:09 ERROR::1: source(getPackageFile("unitTests/runTests.R")) 2016-09-20 08:18:09 ERROR::tools.R/safeExecute: caught exception: 2016-09-20 08:18:09 ERROR::Error in open.connection(file, "rt"): cannot open the connection 2016-09-20 08:18:09 ERROR::tools.R/safeExecute: traceback: 2016-09-20 08:18:09 ERROR::18: (function (e) 2016-09-20 08:18:09 ERROR::17: stop(z$e) 2016-09-20 08:18:09 ERROR::16: safeExecute({ 2016-09-20 08:18:09 ERROR::15: doMergeLanes(indirs) 2016-09-20 08:18:09 ERROR::14: eval(expr, env) 2016-09-20 08:18:09 ERROR::13: try(eval(expr, env), silent = TRUE) 2016-09-20 08:18:09 ERROR::12: safeExecute({ 2016-09-20 08:18:09 ERROR::11: mergeLanes(indirs, mergedir, prepend_str, num_cores = 1, preMergeChecks.do = FALSE, 2016-09-20 08:18:09 ERROR::10: func() 2016-09-20 08:18:09 ERROR::9: system.time(func()) 2016-09-20 08:18:09 ERROR::8: try(system.time(func())) 2016-09-20 08:18:09 ERROR::7: .executeTestCase(funcName, envir = sandbox, setUpFunc = .setUp, 2016-09-20 08:18:09 ERROR::6: .sourceTestFile(testFile, testSuite$testFuncRegexp) 2016-09-20 08:18:09 ERROR::5: runTestSuite(testSuite) 2016-09-20 08:18:09 ERROR::4: eval(expr, envir, enclos) 2016-09-20 08:18:09 ERROR::3: eval(ei, envir) 2016-09-20 08:18:09 ERROR::2: withVisible(eval(ei, envir)) 2016-09-20 08:18:09 ERROR::1: source(getPackageFile("unitTests/runTests.R")) Timing stopped at: 27.228 19.408 54.358 Error in open.connection(file, "rt") : cannot open the connection done successfully. Executing test function test.markDuplicates ... Timing stopped at: 0 0 0.001 Error in DEACTIVATED("Skipped markDuplicates() test") : Skipped markDuplicates() test done successfully. Executing test function test.markDuplicates_w_outfile ... Timing stopped at: 0 0 0 Error in DEACTIVATED("Skipped markDuplicates() test") : Skipped markDuplicates() test done successfully. Executing test function test.preprocessReads ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:18:12 INFO::preprocessReads.R/preprocessReads: starting... 2016-09-20 08:18:12 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:18:12 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq 2016-09-20 08:18:12 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:18:14 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:18:14 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/chunks/chunk_000001/logs/progress.log 2016-09-20 08:18:16 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.044 minutes 2016-09-20 08:18:16 DEBUG::tools.R/processChunks: done 2016-09-20 08:18:16 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 08:18:16 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/results/test_pe.adapter_contaminated_1.RData 2016-09-20 08:18:16 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ... 2016-09-20 08:18:16 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/results/test_pe.adapter_contaminated_2.RData 2016-09-20 08:18:16 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75 2016-09-20 08:18:16 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/results/test_pe.summary_preprocess.tab 2016-09-20 08:18:16 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/bams/processed.aligner_input_1.fastq ... 2016-09-20 08:18:17 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/bams/processed.aligner_input_2.fastq ... 2016-09-20 08:18:17 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/reports/shortReadReport_1 ... 2016-09-20 08:18:19 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.preprocessReads.79f52a80f78d/reports/shortReadReport_2 ... 2016-09-20 08:18:21 INFO::preprocessReads.R/preprocessReads: done done successfully. Executing test function test.preprocessReads.minichunks ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:18:23 INFO::preprocessReads.R/preprocessReads: starting... 2016-09-20 08:18:23 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:18:23 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_2.fastq 2016-09-20 08:18:23 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:18:25 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:18:25 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/chunks/chunk_000001/logs/progress.log 2016-09-20 08:18:27 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.045 minutes 2016-09-20 08:18:27 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/chunks/chunk_000002/logs/progress.log 2016-09-20 08:18:30 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.045 minutes 2016-09-20 08:18:30 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/chunks/chunk_000003/logs/progress.log 2016-09-20 08:18:33 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.044 minutes 2016-09-20 08:18:33 DEBUG::tools.R/processChunks: done 2016-09-20 08:18:33 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 08:18:33 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/results/test_pe.adapter_contaminated_1.RData 2016-09-20 08:18:33 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ... 2016-09-20 08:18:33 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/results/test_pe.adapter_contaminated_2.RData 2016-09-20 08:18:33 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75 2016-09-20 08:18:33 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/results/test_pe.summary_preprocess.tab 2016-09-20 08:18:33 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/bams/processed.aligner_input_1.fastq ... 2016-09-20 08:18:33 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/bams/processed.aligner_input_2.fastq ... 2016-09-20 08:18:33 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/reports/shortReadReport_1 ... 2016-09-20 08:18:35 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.preprocessReads.minichunks.79f52818e558/reports/shortReadReport_2 ... 2016-09-20 08:18:37 INFO::preprocessReads.R/preprocessReads: done done successfully. Executing test function test.preprocessReads_single_end ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:18:40 INFO::preprocessReads.R/preprocessReads: starting... 2016-09-20 08:18:40 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/unit_tests_1.fastq 2016-09-20 08:18:40 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:18:41 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:18:41 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp8SYYBa/test.preprocessReads_single_end.79f511b69d35/chunks/chunk_000001/logs/progress.log 2016-09-20 08:18:44 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.04 minutes 2016-09-20 08:18:44 DEBUG::tools.R/processChunks: done 2016-09-20 08:18:44 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 08:18:44 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads_single_end.79f511b69d35/results/test_se.adapter_contaminated_1.RData 2016-09-20 08:18:44 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75 2016-09-20 08:18:44 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.preprocessReads_single_end.79f511b69d35/results/test_se.summary_preprocess.tab 2016-09-20 08:18:44 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp8SYYBa/test.preprocessReads_single_end.79f511b69d35/bams/processed.aligner_input_1.fastq ... 2016-09-20 08:18:44 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp8SYYBa/test.preprocessReads_single_end.79f511b69d35/reports/shortReadReport_1 ... 2016-09-20 08:18:46 INFO::preprocessReads.R/preprocessReads: done done successfully. Executing test function test.readRNASeqEnds ... done successfully. Executing test function test.readRNASeqEnds.dupmark ... done successfully. Executing test function test.how_many ... done successfully. Executing test function test.plotDF ... done successfully. Executing test function test.relativeBarPlot ... done successfully. Executing test function test.runPipeline ... checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:18:52 INFO::preprocessReads.R/preprocessReads: starting... 2016-09-20 08:18:52 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/extdata/H1993_TP53_subset2500_1.fastq.gz 2016-09-20 08:18:52 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/extdata/H1993_TP53_subset2500_2.fastq.gz 2016-09-20 08:18:52 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:18:53 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:18:53 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log 2016-09-20 08:18:56 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.047 minutes 2016-09-20 08:18:56 DEBUG::tools.R/processChunks: done 2016-09-20 08:18:56 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ... 2016-09-20 08:18:56 INFO::io.R/saveWithID: saving file= test/results/test.adapter_contaminated_1.RData 2016-09-20 08:18:56 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ... 2016-09-20 08:18:56 INFO::io.R/saveWithID: saving file= test/results/test.adapter_contaminated_2.RData 2016-09-20 08:18:56 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=2500 highqual_reads=2500 adapter_contam=0 read_length=100 rRNA_contam_reads=0 processed_reads=2500 input_min_read_length=100 input_max_read_length=100 processed_min_read_length=71 processed_max_read_length=100 2016-09-20 08:18:56 INFO::io.R/saveWithID: saving file= test/results/test.summary_preprocess.tab 2016-09-20 08:18:56 INFO::preprocessReads.R/mergePreprocessedReads: merging file= test/bams/processed.aligner_input_1.fastq ... 2016-09-20 08:18:56 INFO::preprocessReads.R/mergePreprocessedReads: merging file= test/bams/processed.aligner_input_2.fastq ... 2016-09-20 08:18:56 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= test/reports/shortReadReport_1 ... 2016-09-20 08:18:58 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= test/reports/shortReadReport_2 ... 2016-09-20 08:19:00 INFO::preprocessReads.R/preprocessReads: done 2016-09-20 08:19:01 INFO::alignReads.R/alignReads: starting alignment... 2016-09-20 08:19:01 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:19:02 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:19:02 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log 2016-09-20 08:19:07 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.081 minutes 2016-09-20 08:19:07 DEBUG::tools.R/processChunks: done 2016-09-20 08:19:07 INFO::alignReads.R/mergeBAMsAcrossDirs: starting... 2016-09-20 08:19:07 INFO::alignReads.R/mergeBAMsAcrossDirs: done 2016-09-20 08:19:07 INFO::io.R/saveWithID: saving file= test/results/test.summary_alignment.tab 2016-09-20 08:19:07 INFO::io.R/saveWithID: saving file= test/results/test.summary_analyzed_bamstats.tab 2016-09-20 08:19:07 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report... 2016-09-20 08:19:07 INFO::io.R/saveWithID: saving file= test/results/test.summary_target_lengths.tab 2016-09-20 08:19:07 INFO::reportPipelineQA.R/writePreprocessAlignReport: done 2016-09-20 08:19:07 INFO::alignReads.R/alignReads: done 2016-09-20 08:19:08 INFO::countGenomicFeatures.R/countGenomicFeatures: starting... 2016-09-20 08:19:08 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:19:09 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:19:09 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log 2016-09-20 08:19:12 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.041 minutes 2016-09-20 08:19:12 DEBUG::tools.R/processChunks: done 2016-09-20 08:19:12 INFO::countGenomicFeatures.R/mergeCounts: starting... 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.counts_exon.tab 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.counts_gene.tab 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.counts_gene_coding.tab 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.counts_gene_exonic.tab 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.counts_intergenic.tab 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.counts_intron.tab 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.counts_transcript.tab 2016-09-20 08:19:12 INFO::countGenomicFeatures.R/mergeCounts: done 2016-09-20 08:19:12 INFO::io.R/saveWithID: saving file= test/results/test.summary_counts.tab 2016-09-20 08:19:12 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: creating report of genomic features counts... 2016-09-20 08:19:12 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: done 2016-09-20 08:19:12 INFO::countGenomicFeatures.R/countGenomicFeatures: done... 2016-09-20 08:19:12 INFO::coverage.R/calculateCoverage: starting... 2016-09-20 08:19:12 DEBUG::tools.R/processChunks: starting... 2016-09-20 08:19:14 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ... 2016-09-20 08:19:14 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log 2016-09-20 08:19:16 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.039 minutes 2016-09-20 08:19:16 DEBUG::tools.R/processChunks: done 2016-09-20 08:19:16 INFO::io.R/saveWithID: saving file= test/results/test.coverage.RData 2016-09-20 08:19:16 INFO::coverage.R/saveCoverage: saving file= test/results/test.coverage.bw 2016-09-20 08:19:17 INFO::io.R/saveWithID: saving file= test/results/test.summary_coverage.tab 2016-09-20 08:19:17 INFO::coverage.R/calculateCoverage: done 2016-09-20 08:19:17 INFO::analyzeVariants/analyzeVariants: starting ... 2016-09-20 08:19:17 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants... 2016-09-20 08:19:19 INFO::analyzeVariants.R/wrap.callVariants: calling variants... 2016-09-20 08:19:19 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants... 2016-09-20 08:19:20 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants... 2016-09-20 08:19:20 INFO::io.R/saveWithID: saving file= test/results/test.raw_variants.RData 2016-09-20 08:19:20 INFO::io.R/saveWithID: saving file= test/results/test.filtered_variants.RData 2016-09-20 08:19:20 INFO::analyzeVariants.R/wrap.callVariants: ...done 2016-09-20 08:19:20 INFO::analyzeVariants.R/writeVCF: writing vcf file... 2016-09-20 08:19:20 INFO::analyzeVariants.R/writeVCF: ...done 2016-09-20 08:19:20 INFO::io.R/saveWithID: saving file= test/results/test.summary_variants.tab 2016-09-20 08:19:20 INFO::analyzeVariants/analyzeVariants: done 2016-09-20 08:19:20 INFO::Pipeline run successful. done successfully. Executing test function test.calculateTargetLength ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp8SYYBa/tp53-config.txt checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/config/default-config.txt 2016-09-20 08:19:22 INFO::io.R/saveWithID: saving file= /tmp/Rtmp8SYYBa/test.calcTargetLengths.79f55d43da88/results/test_pe.summary_target_lengths.tab done successfully. Executing test function test.sclapply ... Timing stopped at: 1.484 51.12 28.935 Error in mcfork(detached) : unable to fork, possible reason: Cannot allocate memory done successfully. Executing test function test.tryKeepTraceback ... done successfully. Executing test function test.truncateReads ... done successfully. Executing test function test.truncateReads.trim5 ... done successfully. RUNIT TEST PROTOCOL -- Tue Sep 20 08:19:53 2016 *********************************************** Number of test functions: 63 Number of deactivated test functions: 9 Number of errors: 3 Number of failures: 0 1 Test Suite : HTSeqGenie unit testing - 63 test functions, 3 errors, 0 failures ERROR in test.annotateVariants: Error in annotateVariants(vcf) : variant_effect_predictor.pl failed.--vcf --sift b --polyphen b --protein --check_existing --check_alleles --symbol --canonical --ccds --maf_1kg --maf_esp --cache --force_overwrite --no_stats --fork 2 --input_file /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/braf_v600.vcf --output_file /tmp/Rtmp8SYYBa/test.annotateVariants.79f52a878016/results/test_pe.variants_vep.vcf DEACTIVATED test.callVariantsGATK: callVariantsGATK() tests need gatk.path option set DEACTIVATED test.callVariantsGATK.withFiltering: callVariantsGATK() tests need gatk.path option set DEACTIVATED test.checkGATKJar: checkGATKJar() test needs gatk.path option set DEACTIVATED test.gatk: gatk() tests need gatk.path option set DEACTIVATED test.realignIndels: test.realignIndels() tests need gatk.path option set DEACTIVATED test.realignIndelsGATK: realignIndelsGATK() tests need gatk.path option set DEACTIVATED test.realignIndelsGATK.parallel: realignIndelsGATK() tests need gatk.path option set ERROR in test.mergeLanes: Error in open.connection(file, "rt") : cannot open the connection DEACTIVATED test.markDuplicates: Skipped markDuplicates() test DEACTIVATED test.markDuplicates_w_outfile: Skipped markDuplicates() test ERROR in test.sclapply: Error in mcfork(detached) : unable to fork, possible reason: Cannot allocate memory Details *************************** Test Suite: HTSeqGenie unit testing Test function regexp: ^test.+ Test file regexp: ^runit.+\.[rR]$ Involved directory: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.alignReads.R test.alignReads: (2 checks) ... OK (23.68 seconds) test.alignReads.sparsechunks: (2 checks) ... OK (15.51 seconds) test.alignReadsOneSingleEnd: (2 checks) ... OK (1.09 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.analyzeVariants.R test.annotateVariants: ERROR !! Error in annotateVariants(vcf) : variant_effect_predictor.pl failed.--vcf --sift b --polyphen b --protein --check_existing --check_alleles --symbol --canonical --ccds --maf_1kg --maf_esp --cache --force_overwrite --no_stats --fork 2 --input_file /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/test-data/braf_v600.vcf --output_file /tmp/Rtmp8SYYBa/test.annotateVariants.79f52a878016/results/test_pe.variants_vep.vcf test.callVariantsVariantTools.genotype: (2 checks) ... OK (28.62 seconds) test.wrap.callVariants: (4 checks) ... OK (1.71 seconds) test.wrap.callVariants.filters: (2 checks) ... OK (3.12 seconds) test.wrap.callVariants.parallel: (1 checks) ... OK (4.77 seconds) test.wrap.callVariants.which: (1 checks) ... OK (1.51 seconds) test.writeVCF.NULL: (1 checks) ... OK (0.75 seconds) test.writeVCF.vcfStat: (4 checks) ... OK (0.96 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.bamUtils.R test.isFirstFragment: (5 checks) ... OK (0 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.buildGenomicFeatures.R test.buildCountsGRangesList: (3 checks) ... OK (533.58 seconds) test.generateSingleGeneDERs: (4 checks) ... OK (388.32 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.calculateCoverage.R test.computeCoverage: (9 checks) ... OK (0.59 seconds) test.isSparse: (5 checks) ... OK (0.07 seconds) test.mergeCoverage: (1 checks) ... OK (0.69 seconds) test.mergeCoverage.sparse: (2 checks) ... OK (0.6 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.checkConfig.R test.checkConfig.analyzeVariants: (5 checks) ... OK (1.86 seconds) test.findTemplate: (5 checks) ... OK (0.01 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.config.R test.checkConfig: (5 checks) ... OK (2.4 seconds) test.checkConfig.alignReads: (5 checks) ... OK (3.77 seconds) test.getConfig: (14 checks) ... OK (0 seconds) test.loadConfig: (2 checks) ... OK (0 seconds) test.parseDCF: (7 checks) ... OK (0 seconds) test.updateConfig: (1 checks) ... OK (0 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.detectAdapterContam.R test.getAdapterSeqs: (5 checks) ... OK (0.08 seconds) test.isAdapter: (5 checks) ... OK (0.04 seconds) test.isAdapter3.primeEnd: (1 checks) ... OK (0.03 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.detectRRNA.R test.detectRRNA: (1 checks) ... OK (2.34 seconds) test.detectRRNA.paired_end: (1 checks) ... OK (2.48 seconds) test.getRRNAIds: (1 checks) ... OK (0.64 seconds) test.getRRNAIds_random: (1 checks) ... OK (0.65 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.filterQuality.R test.filterByLength: (2 checks) ... OK (0.06 seconds) test.isAboveQualityThresh: (3 checks) ... OK (0.05 seconds) test.trimTailsByQuality: (4 checks) ... OK (0.03 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.gatk.R test.callVariantsGATK : DEACTIVATED, callVariantsGATK() tests need gatk.path option set test.callVariantsGATK.withFiltering : DEACTIVATED, callVariantsGATK() tests need gatk.path option set test.checkGATKJar : DEACTIVATED, checkGATKJar() test needs gatk.path option set test.excludeVariantsByRegion: (3 checks) ... OK (0.13 seconds) test.gatk : DEACTIVATED, gatk() tests need gatk.path option set test.realignIndels : DEACTIVATED, test.realignIndels() tests need gatk.path option set test.realignIndelsGATK : DEACTIVATED, realignIndelsGATK() tests need gatk.path option set test.realignIndelsGATK.parallel : DEACTIVATED, realignIndelsGATK() tests need gatk.path option set test_zipUp: (3 checks) ... OK (0 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.io.R test.FastQStreamer.getReads.pefq: (1 checks) ... OK (1.9 seconds) test.FastQStreamer.getReads.pefq.subsample: (1 checks) ... OK (1.99 seconds) test.FastQStreamer.getReads.segz: (1 checks) ... OK (1.86 seconds) test.FastQStreamer.getReads.truncated: (1 checks) ... OK (1.9 seconds) test.FastQStreamer.subsampler.isdeterministic: (3 checks) ... OK (4.02 seconds) test.createTmpDir: (4 checks) ... OK (0 seconds) test.detectQualityInFASTQFile: (3 checks) ... OK (0.1 seconds) test.getObjectFilename: (4 checks) ... OK (0.22 seconds) test.safeUnlink: (2 checks) ... OK (0.44 seconds) test.writeAudit: (0 checks) ... OK (3.29 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.mergeLanes.R test.mergeLanes: ERROR !! Error in open.connection(file, "rt") : cannot open the connection --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.picard.R test.markDuplicates : DEACTIVATED, Skipped markDuplicates() test test.markDuplicates_w_outfile : DEACTIVATED, Skipped markDuplicates() test --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.preprocessReads.R test.preprocessReads: (5 checks) ... OK (10.54 seconds) test.preprocessReads.minichunks: (5 checks) ... OK (15.87 seconds) test.preprocessReads_single_end: (5 checks) ... OK (8.07 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.readRNASeqEnds.R test.readRNASeqEnds: (6 checks) ... OK (0.05 seconds) test.readRNASeqEnds.dupmark: (2 checks) ... OK (0.06 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.reportPipelineQA.R test.how_many: (3 checks) ... OK (0 seconds) test.plotDF: (3 checks) ... OK (0.14 seconds) test.relativeBarPlot: (2 checks) ... OK (0.04 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.runPipeline.R test.runPipeline: (1 checks) ... OK (30.28 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.targetLengths.R test.calculateTargetLength: (4 checks) ... OK (1.88 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.tools.R test.sclapply: ERROR !! Error in mcfork(detached) : unable to fork, possible reason: Cannot allocate memory test.tryKeepTraceback: (3 checks) ... OK (0.01 seconds) --------------------------- Test file: /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/HTSeqGenie/unitTests/runit.trimReads.R test.truncateReads: (7 checks) ... OK (0.03 seconds) test.truncateReads.trim5: (15 checks) ... OK (0.1 seconds) Error in eval(expr, envir, enclos) : runTests.R: failed to run unit tests, see /home/biocbuild/bbs-3.4-bioc/meat/HTSeqGenie.Rcheck/tests/HTSeqGenie-runit.txt for details Calls: source -> withVisible -> eval -> eval Execution halted
HTSeqGenie.Rcheck/00install.out:
* installing *source* package ‘HTSeqGenie’ ... ** R ** inst ** preparing package for lazy loading ** help *** installing help indices ** building package indices ** installing vignettes ** testing if installed package can be loaded * DONE (HTSeqGenie)
HTSeqGenie.Rcheck/HTSeqGenie-Ex.timings:
name | user | system | elapsed | |
HTSeqGenie | 0.000 | 0.000 | 0.001 | |
buildGenomicFeaturesFromTxDb | 0 | 0 | 0 | |
runPipeline | 0.004 | 0.000 | 0.001 | |