## ----echo=FALSE--------------------------------------------------------------- library(BiocStyle) self <- Biocpkg("alabaster.mae") knitr::opts_chunk$set(error=FALSE, warning=FALSE, message=FALSE) ## ----------------------------------------------------------------------------- library(SummarizedExperiment) rna.counts <- matrix(rpois(60, 10), ncol=6) colnames(rna.counts) <- c("disease1", "disease2", "disease3", "control1", "control2", "control3") rownames(rna.counts) <- c("ENSMUSG00000000001", "ENSMUSG00000000003", "ENSMUSG00000000028", "ENSMUSG00000000031", "ENSMUSG00000000037", "ENSMUSG00000000049", "ENSMUSG00000000056", "ENSMUSG00000000058", "ENSMUSG00000000078", "ENSMUSG00000000085") rna.se <- SummarizedExperiment(list(counts=rna.counts)) colData(rna.se)$disease <- rep(c("disease", "control"), each=3) chip.counts <- matrix(rpois(100, 10), ncol=4) colnames(chip.counts) <- c("disease1", "disease2", "control1", "control3") chip.peaks <- GRanges("chr1", IRanges(1:25*100+1, 1:25*100+100)) chip.se <- SummarizedExperiment(list(counts=chip.counts), rowRanges=chip.peaks) library(MultiAssayExperiment) mapping <- DataFrame( assay = rep(c("rnaseq", "chipseq"), c(ncol(rna.se), ncol(chip.se))), # experiment name primary = c(colnames(rna.se), colnames(chip.se)), # sample identifiers colname = c(colnames(rna.se), colnames(chip.se)) # column names inside each experiment ) mae <- MultiAssayExperiment(list(rnaseq=rna.se, chipseq=chip.se), sampleMap=mapping) ## ----------------------------------------------------------------------------- library(alabaster.mae) tmp <- tempfile() meta <- saveObject(mae, tmp) list.files(tmp, recursive=TRUE) ## ----------------------------------------------------------------------------- roundtrip <- readObject(tmp) class(roundtrip) ## ----------------------------------------------------------------------------- sessionInfo()