## ----setup, include = FALSE--------------------------------------------------- knitr::opts_chunk$set( collapse = TRUE, comment = "#>", error = FALSE, warning = FALSE, message = FALSE, crop = NULL ) stopifnot(requireNamespace("htmltools")) htmltools::tagList(rmarkdown::html_dependency_font_awesome()) sce <- readRDS("sce.rds") ## ---- eval=!exists("SCREENSHOT"), include=FALSE------------------------------- # SCREENSHOT <- function(x, ...) knitr::include_graphics(x, dpi = NA) ## ----init--------------------------------------------------------------------- library(iSEE) app <- iSEE(sce, initial=list( FeatureAssayPlot(PanelWidth=6L), FeatureAssayPlot(PanelWidth=6L) )) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-FAP-basic.png") ## ----fexArg------------------------------------------------------------------- app <- iSEE(sce, initial=list( FeatureAssayPlot(YAxisFeatureName="0610009L18Rik"), FeatureAssayPlot(YAxisFeatureName="0610009B22Rik") )) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-FAP-preset-genes.png") ## ----yaxis-------------------------------------------------------------------- app <- iSEE(sce, initial=list( ColumnDataPlot(YAxis="NREADS", PanelWidth=6L, DataBoxOpen=TRUE), ColumnDataPlot(YAxis="TOTAL_DUP", PanelWidth=6L, DataBoxOpen=TRUE) )) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-CDP-basic.png") ## ----xaxis-------------------------------------------------------------------- fex <- FeatureAssayPlot(DataBoxOpen=TRUE, PanelWidth=6L) # Example 1 fex1 <- fex fex1[["XAxis"]] <- "None" # Example 2 fex2 <- fex fex2[["XAxis"]] <- "Column data" fex2[["XAxisColumnData"]] <- "Core.Type" # Example 3a fex3 <- fex fex3[["XAxis"]] <- "Feature name" fex3[["XAxisFeatureName"]] <- "Zyx" # Example 4 (also requires a row statistic table) fex4 <- fex fex4[["XAxis"]] <- "Feature name" fex4[["XAxisFeatureSource"]] <- "RowDataTable1" rex <- RowDataTable(Selected="Ints2", Search="Ints", PanelWidth=12L) # Initialisation app <- iSEE(sce, initial=list(fex1, fex2, fex3, fex4, rex)) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-FAP-xaxis.png") ## ----redDimPlotDefaults-type-------------------------------------------------- app <- iSEE(sce, initial=list( ReducedDimensionPlot(DataBoxOpen=TRUE, Type="TSNE", XAxis=2L, YAxis=1L, PanelWidth=6L) )) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-ReDP-basic.png") ## ----featAssayPlotDefaults-assay---------------------------------------------- app <- iSEE(sce, initial=list( FeatureAssayPlot(DataBoxOpen=TRUE, Assay="logcounts", PanelWidth=6L), FeatureAssayPlot(DataBoxOpen=TRUE, Assay="tophat_counts", PanelWidth=6L) )) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-FAP-assay.png") ## ----ColorByDefaultColor------------------------------------------------------ cdp <- ColumnDataPlot(VisualBoxOpen=TRUE, VisualChoices=c("Color", "Size", "Point", "Text")) cdp2 <- cdp cdp2[["ColorByDefaultColor"]] <- "chocolate3" cdp2[["PointAlpha"]] <- 0.2 cdp2[["PointSize"]] <- 2 cdp2[["Downsample"]] <- TRUE cdp2[["DownsampleResolution"]] <- 150 cdp2[["FontSize"]] <- 2 app <- iSEE(sce, initial=list(cdp, cdp2)) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-CDP-visual.png") ## ----aesthetic-covariate------------------------------------------------------ cdp <- ColumnDataPlot(VisualBoxOpen=TRUE, VisualChoices=c("Color", "Shape"), ColorByColumnData="Core.Type", ShapeByColumnData="Core.Type", ColorBy="Column data", ShapeBy="Column data") cdp2 <- cdp cdp2[["ColorByColumnData"]] <- "TOTAL_DUP" cdp2[["ShapeByColumnData"]] <- "driver_1_s" app <- iSEE(sce, initial=list(cdp, cdp2)) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-CDP-linked-visual.png") ## ----facet-------------------------------------------------------------------- cdp <- ColumnDataPlot(VisualBoxOpen=TRUE, VisualChoices=c("Facet"), FacetRowBy="Column data", FacetRowByColData="driver_1_s", FacetColumnBy="Column data", FacetColumnByColData="Core.Type", PanelWidth=4L) cdp2 <- cdp cdp2[["FacetRowBy"]] <- "None" cdp3 <- cdp cdp3[["FacetColumnBy"]] <- "None" app <- iSEE(sce, initial=list(cdp, cdp2, cdp3)) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-CDP-facets.png") ## ----select-brish------------------------------------------------------------- # Preconfigure the receiver panel cdArgs <- ColumnDataPlot( XAxis="Column data", XAxisColumnData="driver_1_s", # Configuring the selection parameters. SelectionBoxOpen=TRUE, ColumnSelectionSource="ReducedDimensionPlot1", ColorBy="Column selection", # Throwing in some parameters for aesthetic reasons. ColorByDefaultColor="#BDB3B3", PointSize=2, PanelWidth=6L) # Preconfigure the sender panel, including the point selection. # NOTE: You don't actually have to write this from scratch! Just # open an iSEE instance, make a brush and then look at the 'BrushData' # entry when you click on the 'Display panel settings' button. rdArgs <- ReducedDimensionPlot( BrushData = list( xmin = 13.7, xmax = 53.5, ymin = -36.5, ymax = 37.2, coords_css = list(xmin = 413.2, xmax = 650.2, ymin = 83.0, ymax = 344.0), coords_img = list(xmin = 537.2, xmax = 845.3, ymin = 107.9, ymax = 447.2), img_css_ratio = list(x = 1.3, y = 1.3), mapping = list(x = "X", y = "Y"), domain = list(left = -49.1, right = 57.2, bottom = -70.4, top = 53.5), range = list(left = 50.9, right = 873.9, bottom = 603.0, top = 33.1), log = list(x = NULL, y = NULL), direction = "xy", brushId = "ReducedDimensionPlot1_Brush", outputId = "ReducedDimensionPlot1" ), PanelWidth=6L ) app <- iSEE(sce, initial=list(cdArgs, rdArgs)) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-ReDP-select.png") ## ----select-lasso------------------------------------------------------------- # Preconfigure the sender panel, including the point selection. # NOTE: again, you shouldn't try writing this from scratch! Just # make a lasso and then copy the panel settings in 'BrushData'. rdArgs[["BrushData"]] <- list( lasso = NULL, closed = TRUE, panelvar1 = NULL, panelvar2 = NULL, mapping = list(x = "X", y = "Y"), coord = structure(c(18.4, 18.5, 26.1, 39.9, 55.2, 50.3, 54.3, 33.3, 18.4, 35.5, -4.2, -21.2, -46.1, -43.5, -18.1, 7.3, 19.7, 35.5), .Dim = c(9L, 2L) ) ) app <- iSEE(sce, initial=list(cdArgs, rdArgs)) ## ---- echo=FALSE-------------------------------------------------------------- SCREENSHOT("screenshots/configure-ReDP-lasso.png") ## ----------------------------------------------------------------------------- introtour <- defaultTour() head(introtour) ## ----sessioninfo-------------------------------------------------------------- sessionInfo() # devtools::session_info()