Vignette of the a4Classif package
2025-10-07
1 Introduction
This document explains the functionalities available in the a4Classif package.
This package contains for classification of Affymetrix microarray data, stored in an ExpressionSet. This package integrates within the Automated Affymetrix Array Analysis suite of packages.
## Loading required package: a4Core## Loading required package: a4Preproc##
## a4Classif version 1.57.0## Loading required package: Biobase## Loading required package: BiocGenerics## Loading required package: generics##
## Attaching package: 'generics'## The following objects are masked from 'package:base':
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## as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
## setequal, union##
## Attaching package: 'BiocGenerics'## The following objects are masked from 'package:stats':
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## IQR, mad, sd, var, xtabs## The following objects are masked from 'package:base':
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## Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
## as.data.frame, basename, cbind, colnames, dirname, do.call,
## duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
## mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
## rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
## unsplit, which.max, which.min## Welcome to Bioconductor
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## Vignettes contain introductory material; view with
## 'browseVignettes()'. To cite Bioconductor, see
## 'citation("Biobase")', and for packages 'citation("pkgname")'.To demonstrate the functionalities of the package, the ALL dataset is used. The genes are annotated thanks to the addGeneInfo utility function of the a4Preproc package.
data(ALL, package = "ALL")
ALL <- addGeneInfo(ALL)## Loading required package: hgu95av2.db## Loading required package: AnnotationDbi## Loading required package: stats4## Loading required package: IRanges## Loading required package: S4Vectors##
## Attaching package: 'S4Vectors'## The following object is masked from 'package:utils':
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## findMatches## The following objects are masked from 'package:base':
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## I, expand.grid, unname## Loading required package: org.Hs.eg.db## ## ALL$BTtype <- as.factor(substr(ALL$BT,0,1))2 Classify microarray data
2.1 Lasso regression
resultLasso <- lassoClass(object = ALL, groups = "BTtype")
plot(resultLasso,
label = TRUE,
main = "Lasso coefficients in relation to degree of penalization."
)
topTable(resultLasso, n = 15)## The lasso selected 16 genes. The top 15 genes are:
##
## Gene Coefficient
## 38319_at CD3D 0.95966733
## 35016_at CD74 -0.60928095
## 38147_at SH2D1A 0.49240967
## 35792_at MGLL 0.46856925
## 37563_at SRGAP3 0.26648240
## 38917_at YME1L1 0.25100075
## 40278_at GGA2 -0.25017550
## 41164_at IGHM -0.12387272
## 41409_at THEMIS2 -0.10581122
## 38242_at BLNK -0.10309606
## 35523_at HPGDS 0.10169706
## 38949_at PRKCQ 0.07832802
## 33316_at TOX 0.06963509
## 33839_at ITPR2 0.05801832
## 40570_at FOXO1 -0.048588632.2 PAM regression
resultPam <- pamClass(object = ALL, groups = "BTtype")
plot(resultPam,
main = "Pam misclassification error versus number of genes."
)
topTable(resultPam, n = 15)## Pam selected 53 genes. The top 15 genes are:
##
## GeneSymbol B.score T.score av.rank.in.CV prop.selected.in.CV
## 38319_at CD3D -0.8044 2.3156 1 1
## 38147_at SH2D1A -0.4644 1.3369 2 1
## 33238_at LCK -0.3754 1.0808 4.1 1
## 35016_at CD74 0.3753 -1.0804 3.5 1
## 38095_i_at HLA-DPB1 0.3589 -1.0331 5.2 1
## 37039_at HLA-DRA 0.3536 -1.018 5.9 1
## 38096_f_at HLA-DPB1 0.3403 -0.9796 6.7 1
## 2059_s_at LCK -0.3243 0.9336 7.7 1
## 38833_at HLA-DPA1 0.2921 -0.8408 9.2 1
## 41723_s_at <NA> 0.2652 -0.7636 10.9 1
## 1110_at TRDC -0.2599 0.7481 11.2 1
## 38242_at BLNK 0.2387 -0.6871 12.7 1
## 1096_g_at CD19 0.2377 -0.6842 12.5 1
## 37344_at HLA-DMA 0.2303 -0.6631 13.4 1
## 39389_at CD9 0.2211 -0.6366 15 1confusionMatrix(resultPam)## predicted
## true B T
## B 95 0
## T 0 332.3 Random forest
# select only a subset of the data for computation time reason
ALLSubset <- ALL[sample.int(n = nrow(ALL), size = 100, replace = FALSE), ]
resultRf <- rfClass(object = ALLSubset, groups = "BTtype")
plot(resultRf)
topTable(resultRf, n = 15)## Random forest selected 21 genes. The top 15 genes are:
##
## GeneSymbol
## 1061_at IL10RA
## 1688_s_at MNAT1
## 32941_at IRF8
## 33848_r_at CDKN1B
## 34558_at OPRL1
## 34808_at SIK3
## 35260_at MLXIP
## 36795_at PSAP
## 37500_at ZNF175
## 37541_at SELPLG
## 37737_at PCMT1
## 38042_at <NA>
## 38453_at ICAM2
## 39608_at SIM2
## 39838_at CLASP12.4 ROC curve
ROCcurve(gene = "ABL1", object = ALL, groups = "BTtype")## Warning in ROCcurve(gene = "ABL1", object = ALL, groups = "BTtype"): Gene ABL1 corresponds to 6 probesets; only the first probeset ( 1635_at ) has been displayed on the plot.
3 Appendix
3.1 Session information
## R version 4.5.1 Patched (2025-08-23 r88802)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.3 LTS
##
## Matrix products: default
## BLAS: /home/biocbuild/bbs-3.22-bioc/R/lib/libRblas.so
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.12.0 LAPACK version 3.12.0
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_GB LC_COLLATE=C LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_US.UTF-8 LC_NAME=C LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## time zone: America/New_York
## tzcode source: system (glibc)
##
## attached base packages:
## [1] stats4 stats graphics grDevices utils datasets methods base
##
## other attached packages:
## [1] hgu95av2.db_3.13.0 org.Hs.eg.db_3.21.0 AnnotationDbi_1.71.1 IRanges_2.43.5 S4Vectors_0.47.4 ALL_1.51.0 Biobase_2.69.1 BiocGenerics_0.55.1 generics_0.1.4 a4Classif_1.57.0 a4Preproc_1.57.0 a4Core_1.57.0
##
## loaded via a namespace (and not attached):
## [1] sass_0.4.10 varSelRF_0.7-8 shape_1.4.6.1 RSQLite_2.4.3 lattice_0.22-7 digest_0.6.37 evaluate_1.0.5 grid_4.5.1 iterators_1.0.14 fastmap_1.2.0 blob_1.2.4 foreach_1.5.2 jsonlite_2.0.0 glmnet_4.1-10 Matrix_1.7-4 DBI_1.2.3 survival_3.8-3 httr_1.4.7 Biostrings_2.77.2 codetools_0.2-20 jquerylib_0.1.4 cli_3.6.5 crayon_1.5.3
## [24] rlang_1.1.6 XVector_0.49.1 pamr_1.57 bit64_4.6.0-1 splines_4.5.1 cachem_1.1.0 yaml_2.3.10 tools_4.5.1 parallel_4.5.1 memoise_2.0.1 ROCR_1.0-11 vctrs_0.6.5 R6_2.6.1 png_0.1-8 lifecycle_1.0.4 Seqinfo_0.99.2 KEGGREST_1.49.1 randomForest_4.7-1.2 bit_4.6.0 cluster_2.1.8.1 pkgconfig_2.0.3 bslib_0.9.0 Rcpp_1.1.0
## [47] xfun_0.53 knitr_1.50 htmltools_0.5.8.1 rmarkdown_2.30 compiler_4.5.1