Sequence difference plot

Here we use the data published in Potato Research(Chang et al. 2015) as an example.

fas <- list.files(system.file("examples","GVariation", package="seqcombo"),
                  pattern="fas", full.names=TRUE)
fas

## [1] "/tmp/RtmpD9Qfmc/Rinst10ca31d90e4/seqcombo/examples/GVariation/A.Mont.fas"  
## [2] "/tmp/RtmpD9Qfmc/Rinst10ca31d90e4/seqcombo/examples/GVariation/B.Oz.fas"    
## [3] "/tmp/RtmpD9Qfmc/Rinst10ca31d90e4/seqcombo/examples/GVariation/C.Wilga5.fas"

The input fasta file should contains two aligned sequences. User need to specify which sequence (1 or 2, 1 by default) as reference. The seqdiff function will parse the fasta file and calculate the nucleotide differences by comparing the non-reference one to reference.

x1 <- seqdiff(fas[1], reference=1)
x1

## sequence differences of Mont and CF_YL21 
## 1181 sites differ:
##   A   C   G   T 
## 286 315 301 279

We can visualize the differences by plot method:

plot(x1)

We can parse several files and visualize them simultaneously.

x <- lapply(fas, seqdiff)
plts <- lapply(x, plot)
plot_grid(plotlist=plts, ncol=1, labels=LETTERS[1:3])

Sequence similarity plot

fas <- system.file("examples/GVariation/sample_alignment.fa", package="seqcombo")
simplot(fas, 'CF_YL21')

Session info

Here is the output of sessionInfo() on the system on which this document was compiled:

## R version 3.6.0 (2019-04-26)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 18.04.2 LTS
## 
## Matrix products: default
## BLAS:   /home/biocbuild/bbs-3.9-bioc/R/lib/libRblas.so
## LAPACK: /home/biocbuild/bbs-3.9-bioc/R/lib/libRlapack.so
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
## [1] igraph_1.2.4.1  ggplot2_3.1.1   emojifont_0.5.2 tibble_2.1.1   
## [5] seqcombo_1.6.0 
## 
## loaded via a namespace (and not attached):
##  [1] Rcpp_1.0.1          compiler_3.6.0      pillar_1.3.1       
##  [4] plyr_1.8.4          XVector_0.24.0      sysfonts_0.8       
##  [7] prettydoc_0.2.1     tools_3.6.0         zlibbioc_1.30.0    
## [10] digest_0.6.18       evaluate_0.13       gtable_0.3.0       
## [13] pkgconfig_2.0.2     rlang_0.3.4         rvcheck_0.1.3      
## [16] yaml_2.2.0          parallel_3.6.0      xfun_0.6           
## [19] proto_1.0.0         withr_2.1.2         showtextdb_2.0     
## [22] stringr_1.4.0       dplyr_0.8.0.1       knitr_1.22         
## [25] Biostrings_2.52.0   S4Vectors_0.22.0    IRanges_2.18.0     
## [28] stats4_3.6.0        grid_3.6.0          cowplot_0.9.4      
## [31] tidyselect_0.2.5    glue_1.3.1          R6_2.4.0           
## [34] rmarkdown_1.12      purrr_0.3.2         magrittr_1.5       
## [37] scales_1.0.0        htmltools_0.3.6     BiocGenerics_0.30.0
## [40] showtext_0.6        assertthat_0.2.1    colorspace_1.4-1   
## [43] labeling_0.3        stringi_1.4.3       lazyeval_0.2.2     
## [46] munsell_0.5.0       crayon_1.3.4